Paulinella micropora KR01 C4 pathway RNA-seq experiment - High Light ... - SRA

SRX15663453: Paulinella micropora KR01 C4 pathway RNA-seq experiment - High Light elevated CO2 treatment 3
1 ILLUMINA (Illumina HiSeq 2500) run: 32.5M spots, 9.8G bases, 3Gb downloads

Design: Paulinella micropora KR01 cells were grown at 26C in Fernbach flasks containing 0.3 L DY-V medium on a 16 h:8 h light/dark cycle at a light intensity of roughly 15-20 micromole photons m-2 s-1. Cells were shifted to a light intensity of 150 umol photons m2 s1 and supplemented with sodium bicarbonate (NaHCO3; final concentration of 5mM) at the beginning of the light cycle and left for 6 hours before RNA was harvested. Cells were centrifuged at 4000rpm for 10 min at 4C. The resulting pellets were frozen in liquid nitrogen and stored at -80C until RNA was extracted using a Qiagen RNeasy Plant Mini Kit following the manufacturers instructions (Qiagen, Hilden, Germany). RNA was eluted with 50 ul RNAase-free water and quantified using a Qubit. Library construct and RNA-sequencing was performed by Genewiz.

Submitted by: Rutgers University

Study: Paulinella micropora KR01 C4 RNA-seq experiment

PRJNA847773 • SRP379491 • All experiments • All runs

show Abstracthide Abstract

The rhizarian amoeba Paulinella harbors an organelle derived from a cyanobacterial endosymbiont, making it the only recent example of primary plastid endosymbiosis. The genes required for a C4 carbon fixation cycle are present in KR01 but their response to stimuli (such as high light or excess CO2) has yet to be described. This project seeks to explore the expression of these C4-releated genes by exposing cultures of KR01 to various stimuli that should result in predictable patterns of up or downregulation.

Sample: Paulinella micropora KR01 C4 RNA-seq experiment high light and added bicarbonate

SAMN28956602 • SRS13361848 • All experiments • All runs

Organism: Paulinella micropora

Library:

Name: Paulinella micropora KR01 C4 pathway RNA-seq experiment - HLBC3

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 32.5M spots, 9.8G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR19612681	32,537,721	9.8G	3Gb	2023-11-30

ID:: 22286823

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